High Content Monomer

Plant extraction process

1. Select plants/medicinal materials. It is nothing more than ancient prescriptions, proven prescriptions, and folk medicinal herbs. At present, common and uncommon medicinal materials have been studied. Most of the time, the amount of medicinal materials has been increased to extract low-isolated components, or medicinal plants have not been studied from Miao medicine, Tibetan medicine, Mongolian medicine, Africa, Latin America and other places. 

2. Extract. Solvent petroleum ether, n-hexane, cyclohexane, benzene, chloroform, ethyl acetate, n-butanol, acetone, ethanol, methanol, water (small polarity → large polarity). Daily decoction of medicines is effective, use water and ethanol and other solvents with high polarity. Artemisinin and other boiling methods are not effective, use petroleum ether and other solvents with low polarity. The common medicinal materials, water/alcohol/ether, are presented again, and more compounds can be separated and identified.

3. Separation. This is the most important task. There are dozens of compounds in the solution extracted in the second step. Generally, column chromatography is used, which is what we often call column flushing. The workload is large, boring, and low-tech. A master's degree may do this every day for 2 years of experimentation. As shown in the figure below, the column for separating compounds is as large as 2 meters high and as small as 10 cm. Change the solvent conditions of the mobile phase, change the material of the column, and repeatedly wash the column under different conditions and separation principles to separate the monomer compound.